What Is the Best Time and Dose to Take NAD+?

NAD+ At Home Kit

As women move through their 50s and beyond, staying energized, sharp, and balanced becomes a greater focus. But if you're constantly feeling low on energy or struggling with brain fog, the issue may not just be hormones or age—it could be your NAD+ levels.

NAD+ (nicotinamide adenine dinucleotide) is a vital coenzyme that fuels everything from cellular energy to DNA repair. As we age, NAD+ levels naturally decline, impacting everything from metabolism to mood. The good news? With the right supplementation routine, you can replenish those levels—and start feeling more like yourself again.

In this guide, we’ll dive into the best time and dose to take NAD+, especially for women over 50, and how to get the most out of your supplements—whether in capsule, powder, or injection form.

Why NAD+ Is a Game Changer for Women Over 50

By the time you hit your 50s, your NAD+ levels may be half of what they were in your twenties. Combined with the hormonal shifts of menopause, this drop can accelerate aging and impact nearly every part of your well-being.

NAD+ supplementation may help:

• Boost energy and reduce daily fatigue

• Sharpen memory and improve mental clarity

• Support hormonal balance and libido

• Promote restful sleep and reduce anxiety

• Enhance skin elasticity and cellular repair

• Reverse signs of biological aging triggered by menopause

  
  

To experience these benefits fully, timing and dosage matter more than most realize.

The Best Time to Take NAD+ for Maximum Impact

Choosing when to take your NAD+ supplement can make a big difference in how you feel and how your body responds. Here’s what to consider:

Morning

• Most recommended timing

• Aligns with your body’s natural circadian rhythm

• Supports daytime energy, metabolism, and alertness

  
  

Pre-Workout

• Ideal for those prioritizing athletic performance or mitochondrial health

• Take 30–60 minutes before exercise for a natural energy boost

  
  

Evening

• Some prefer nighttime dosing to support cellular repair during sleep

• However, energy-boosting effects may interfere with sleep for some

  
  

Best Practice

• Take NAD+ on an empty stomach—either first thing in the morning or 2+ hours after your last meal

• Consistency is key: daily use maintains stable NAD+ levels over time

Finding the Right Dosage for Your Needs

The best time and dose to take NAD+ depends on the type of supplement and your individual goals. Here’s a breakdown of common precursors and direct NAD+ options:

Nicotinamide Riboside (NR)

• Daily range: 100–1000 mg

• Most commonly used dose: 250–500 mg

• Known for improving energy, metabolism, and neurological function

  
  

Nicotinamide Mononucleotide (NMN)

• Daily range: 150–1200 mg

• Effective range for most users: 250–900 mg

• Popular among women for its anti-aging and metabolic benefits

  
  

Nicotinamide (NAM)

• Well-tolerated up to 3g per day

• Significant NAD+ level increases seen at 900 mg

• A more budget-friendly option, but may inhibit certain NAD+ pathways in high doses

Understanding NAD+ Injection Dosage Per Day

For those seeking faster and more bioavailable results, NAD+ injections are a potent at-home option—especially appealing to women managing fatigue, brain fog, or accelerated aging.

Here’s what you need to know about NAD+ injection dosage per day:

Subcutaneous NAD+ Injections

• Standard dose: 50–200 mg per injection

• Common protocol:

  • 50 mg daily for the first 3 days

  • Then every other day for maintenance

• Benefits include rapid energy boost, improved sleep, and enhanced mental clarity

  
  

IV Therapy (in clinics)

• Doses typically range from 500–1500 mg per session

• Reserved for higher-intensity protocols or medical settings

  
  

Best Practices for Injections

• Administer in the abdomen or upper thigh

• Rotate injection sites

• Store vials refrigerated for maximum potency

• Use clean, sterile technique and follow product instructions carefully

Popular At-Home NAD+ Injection Kits

If convenience is key, at-home kits are a safe and effective way to stay on track. Here are two of the most reputable options:

NAD+ At-Home Injection Kit

• 1000 mg NAD+ in 5 ml vial

• Includes 20 doses of 50 mg each

• Kit includes micro-needles, antiseptics, and a sharps bin

• Lasts ~5 weeks with alternating-day protocol

  
  

MitoGive NAD+ 1500mg Kit

• 30-day supply with pre-filled syringes

• Ideal for daily, consistent supplementation

• Includes 1500 mg NAD+ and sterile equipment

How to Get Started Safely

Whether you’re choosing oral supplements or injectable NAD+, follow these guidelines to stay safe and maximize benefits:

• Start with a lower dose to assess tolerance

• Choose high-quality products from reputable brands

• Store injectables in the refrigerator

• Avoid taking with high-fat meals (which may affect absorption)

• Discuss your plan with a healthcare provider, especially if you have underlying health conditions or take medications

• Discontinue use if you experience any adverse side effects like nausea or dizziness

Your Personalized NAD+ Plan

There’s no one-size-fits-all approach—but when it comes to the best time and dose to take NAD+, the key is alignment with your body’s rhythm and staying consistent.

For most women over 50:

• Take 250–500 mg of NMN or NR in the morning on an empty stomach

• If opting for injections, start with 50 mg daily for 3 days, then switch to every other day

• Combine NAD+ with a healthy diet, quality sleep, and moderate exercise for best results

Final Word

Replenishing your NAD+ levels can help you reclaim energy, sharpen your focus, and feel more balanced as you navigate life after 50. Whether you're using oral supplements or at-home injections, understanding the best time and dose to take NAD+ will help you get the most from your routine.

As always, partner with a knowledgeable healthcare professional to fine-tune your dosage and ensure your approach is tailored to your needs.

FAQ: Best Time and Dose to Take NAD+

Is it better to take NAD+ in the morning or at night? 

Morning is generally best. Taking NAD+ early in the day aligns with your body’s natural energy cycle and helps support metabolism and alertness. However, some prefer nighttime dosing to support cellular repair—just be cautious of any sleep interference.

Should I take NAD+ with food? 

For optimal absorption, it’s recommended to take NAD+ supplements on an empty stomach, ideally first thing in the morning or at least 2 hours after a meal.

What is the best NAD+ dosage for beginners? 

Start low to assess your tolerance. For most women over 50, a daily dose of 250–500 mg of NR or NMN is effective. If using injections, 50 mg per day for the first 3 days is standard, followed by every other day for maintenance.

What’s the recommended NAD+ injection dosage per day? 

The typical subcutaneous NAD+ injection dosage per day ranges from 50 to 200 mg. Most protocols suggest 50 mg daily for 3 days, then switching to alternate days for ongoing support.

How long does it take to see results from NAD+? 

Some users report noticeable energy and focus improvements within a few days. However, benefits such as improved sleep, mood, and skin elasticity may take several weeks of consistent use.

Can NAD+ help with menopause symptoms? 

Yes. NAD+ may support hormonal balance, brain function, mood, and sleep—all commonly impacted during menopause. Many women over 50 find it helpful for managing fatigue and age-related decline.